Fig 1: Serpinc1 expression inhibited macrophage M2 immune infiltration in hepatocellular carcinoma. (A–K) Analysis of immune cell infiltration abundance in hepatocellular carcinoma and normal controls with CIBERSORT. Myeloid dendritic cell resting (A), Monocyte (B), Macrophage M0 (C), Macrophage M1 (D), Macrophage M2 (E), Mast cell resting (F), Mast cell activated (G), T cell regulatory (H), T cell follicular helper (I), T cell gamma delta (J), and Neutrophil (K). (L–O) Analysis of correlation between lymphocyte and outcomes in hepatocellular carcinoma patients from TIMER2.0 database. Macrophage (L), macrophage 0 (M), macrophage 2 (N), and macrophage/monocyte (O). (P1–P4) Analysis of correlation between serpinc1 and immune infiltration from TISIDB database. Macrophage (P1), monocyte (P2), NK CD56dim (P3), and NK (P4). (Q, R) THP1 cells activated by 50 nM PMA were co-cultured with HepG2 overexpressed control (blue) or serpinc1 (yellow) or alone (red). Macrophage M2 marker CD163 and M1 marker CD80 were detected by flow cytometry. Representative image (Q) and statistical analysis (R). (S–V) HepG2 cells were overexpressed control or serpinc1, and analyzed the macrophage M2-associated factors in culture medium. Statistical analysis of IL4 (S), IL13 (T), IL 10 (U), and lactate (V). *P < 0.05, ns, no significant, P > 0.05.
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